Estrogen Receptor-α (Phospho-Tyr537) Antibody

Catalog Number:

11657

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P03372;
NCBI Gene#: 2099;
NCBI Protein#: NP_000116.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 537(P-L-Y(p)-D-L) derived from Human Estrogen Receptor-α.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of ER-α only when phosphorylated at tyrosine 537.

Applications:

WB IHC

Background:

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

Sehime Temel, Endocrinology, Oct 2002; 143: 3974.
Xin-Tian Zhang, AACR Meeting Abstracts, Apr 2005; 2005: 302.
Suwit J. Somponpun, Am J Physiol Regulatory Integrative Comp Physiol, Sep 2004; 287: R661 - R669.
Marcello Maggiolini, Cancer Res., Oct 1999;

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF