CD3 ζ (Phospho-Tyr142) Antibody

Catalog Number:

11655

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P20963;
NCBI Gene#: 919;
NCBI Protein#: NP_000725.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 142 (G-L-Y(p)-Q-G) derived from Human CD3 ζ.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of CD3 ζ only when phosphorylated at tyrosine 142.

Applications:

WB

Background:

Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.

Linda A. Trimble, Blood, Aug 2000; 96: 1021 - 1029.
Sandeep Krishnan, J. Immunol., Sep 2005; 175: 3417 - 3423.
Linda A. Trimble, J. Virol., Aug 2000; 74: 7320 - 7330.
Hisashi Arase, J. Immunol., Jan 2001; 166: 21.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF