Arrestin 1 (Phospho-Ser412) Antibody

Catalog Number:

11654

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P49407;
NCBI Gene#: 408;
NCBI Protein#: NP_004032.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 412 (T-G-S(p)-P-Q) derived from Human Arrestin 1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Arrestin-1 only when phosphorylated at serine 412.

Applications:

WB IHC

Background:

Functions in regulating agonist-mediated G-protein coupled receptor (GPCR) signaling by mediating both receptor desensitization and resensitization processes. During homologous desensitization, beta-arrestins bind to the GPRK-phosphorylated receptor and sterically preclude its coupling to the cognate G-protein; the binding appears to require additional receptor determinants exposed only in the active receptor conformation.

Greg Buchanan. AACR Meeting Abstracts, Apr 2006; 2006: 430.
D. Alex Groarke. Mol. Pharmacol., Feb 2001; 59: 375.
Karen McConalogue. Mol. Biol. Cell, Aug 1998; 9: 2305.
Maria Stella Lombardi. Stroke, Apr 2004; 35: 981 - 986.

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF