Product Datasheet  
Arrestin 1 (Phospho-Ser412) Antibody  
Catalog Number: 11654  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P49407;
    NCBI Gene#: 408;
    NCBI Protein#: NP_004032.2.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Serine 412 (T-G-S(p)-P-Q) derived from Human Arrestin 1.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100

  • other_names：  
  • ARR1; arrestin 2; arrestin beta 1; beta-arrestin-1;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of Arrestin-1 only when phosphorylated at serine 412.
  • Applications：  
  • WB IHC
  • Background：  

  • Functions in regulating agonist-mediated G-protein coupled receptor (GPCR) signaling by mediating both receptor desensitization and resensitization processes. During homologous desensitization, beta-arrestins bind to the GPRK-phosphorylated receptor and sterically preclude its coupling to the cognate G-protein; the binding appears to require additional receptor determinants exposed only in the active receptor conformation.

    Greg Buchanan. AACR Meeting Abstracts, Apr 2006; 2006: 430.
    D. Alex Groarke. Mol. Pharmacol., Feb 2001; 59: 375.
    Karen McConalogue. Mol. Biol. Cell, Aug 1998; 9: 2305.
    Maria Stella Lombardi. Stroke, Apr 2004; 35: 981 - 986.




 
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