p130 Cas (Phospho-Tyr410) Antibody

Catalog Number:

11651

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P56945;
NCBI Gene#: 9564;
NCBI Protein#: NP_055382.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 410(G-V-Y(p)-A-V) derived from Human p130 Cas .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of p130 Cas only when phosphorylated at tyrosine 410.

Applications:

WB IHC

Background:

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.

Katarzyna Modzelewska, J. Biol. Chem., Dec 2006; 281: 37527 - 37535.
Kevin Ogden, Am J Physiol Heart Circ Physiol, Dec 2006; 291: H2857 - H2863.
Sangkil Nam, Cancer Res., Oct 2005; 65: 9185 - 9189.
Norikazu Yamana, Mol. Cell. Biol., Sep 2006; 26: 6844 - 6

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF