C/EBP-α (Phospho-Ser21) Antibody

Catalog Number:

11648

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P49715;
NCBI Gene#: 1050;
NCBI Protein#: NP_004355.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 21 (L-Q-S(p)-P-P) derived from Human C/EBP-α.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of C/EBP-α only when phosphorylated at serine 21.

Applications:

WB IHC

Background:

C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

Takashi Akasaka, Blood, Apr 2007; 109: 3451 - 3461.
Carolina Gillio-Meina, Biol Reprod, May 2005; 72: 1194 - 1204.
Rapha?l M¨¦tivier, Mol. Endocrinol., Nov 2001; 15: 1953.
Neoncheol Jung, Eur. J. Biochem., Oct 2000; 267: 6180.

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF