Product Datasheet  
C/EBP-α (Phospho-Ser21) Antibody  
Catalog Number: 11648  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P49715;
    NCBI Gene#: 1050;
    NCBI Protein#: NP_004355.2.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Serine 21 (L-Q-S(p)-P-P) derived from Human C/EBP-α.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  

  • Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100

  • other_names：  
  • CAP4; ICE8; MACH; MCH5; Mch-5
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of C/EBP-α only when phosphorylated at serine 21.
  • Applications：  
  • WB IHC
  • Background：  

  • C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

    Takashi Akasaka, Blood, Apr 2007; 109: 3451 - 3461.
    Carolina Gillio-Meina, Biol Reprod, May 2005; 72: 1194 - 1204.
    Rapha?l M¨¦tivier, Mol. Endocrinol., Nov 2001; 15: 1953.
    Neoncheol Jung, Eur. J. Biochem., Oct 2000; 267: 6180.




 
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