Synaptotagmin (Phospho-Thr202) Antibody

Catalog Number:

11645

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P21579;
NCBI Gene#: 6857;
NCBI Protein#: NP_001129277.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 202(R-K-T(p)-L-N) derived from Human Synaptotagmin.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Synaptotagmin only when phosphorylated at threonine 202.

Applications:

WB IHC

Background:

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse.

E Carafoli, Crit. Rev. Biochem. Mol. Biol., Apr 2001; 36: 107.
Gry Houeland, J Neurophysiol, Jan 2007; 97: 134 - 143.
Christelle Carteron, J. Cell Sci., Mar 2006; 119: 898 - 909.
Vladimir I. Slepnev, Science, Aug 1998; 281: 821.

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF