PHB(Phospho-Thr258) Antibody

Catalog Number:

11588

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P35232;
NCBI Gene ID: 5245;
NCBI Protein: NP_001268425.1.

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 258 (N-I-T(p)-Y-L) derived from Human PHB.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of PHB only when phosphorylated at threonine 258.

Applications:

WB

Background:

 Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. 

References:

appl_detail:

 Western blotting: 1:500~1:1000

Datasheet PDF