Product Datasheet  
PHB(Phospho-Thr258) Antibody  
Catalog Number: 11588  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species:  
  • Rabbit
  • Amount:  
  • 100μgμg
  • Swiss-Prot No.:  
  • Swiss-Prot: P35232;
    NCBI Gene ID: 5245;
    NCBI Protein: NP_001268425.1.
  • Form of Antibody:  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage:  
  • Store at -20°C
  • Immunogen:  
  • Peptide sequence around phosphorylation site of threonine 258 (N-I-T(p)-Y-L) derived from Human PHB.
  • reactivity:  
  • Hu Ms Rt
  • appl_detail:  
  •  Western blotting: 1:500~1:1000

  • other_names:  
  • Prohibitin; PHB1
  • Purification:  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity:  
  • The antibody detects endogenous level of PHB only when phosphorylated at threonine 258.
  • Applications:  
  • WB
  • Background:  
  •  Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. 




 
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