TrkA(Phospho-Tyr791) Antibody

Catalog Number:

11326

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P04629
NCBI Protein: NP_001007793.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine791 (P-V-Y(p)-L-D) derived from Human TrkA.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of TrkA only when phosphorylated at tyrosine 791.

Applications:

WB IF

Background:

Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway.

Wiese S, et al. Proc Natl Acad Sci U S A. 2007 Oct 23; 104(43):17210-5.
Valdez G, et al. Proc Natl Acad Sci U S A. 2007 Jul 24;104(30):12270-5
Inoue K, et al. J Biol Chem. 2007 Aug 17;282(33):24175-84

References:

appl_detail:

Predicted MW: 140kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Datasheet PDF