SHP-1(Phospho-Tyr536) Antibody

Catalog Number:

11318

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P29350
NCBI Protein: NP_002822.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 536 (S-E-Y(p)-G-N) derived from Human SHP-1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of SHP-1 only when phosphorylated at tyrosine 536.

Applications:

WB IHC IF

Background:

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.

Migone TS, et al. (1998) Proc Natl Acad Sci USA; 95(7): 3845-3850.
Timms JF, et al.(1998) Mol Cell Biol; 18(7): 3838-3850.
Kanagasundaram V, et al. (1999) Mol Cell Biol; 19(6): 4079-4092.
Hauck CR, et al. (1999) Infect Immun; 67(10): 5490-5494.

References:

appl_detail:

Predicted MW: 68kd

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

 

Datasheet PDF