Gab1(Phospho-Tyr627) Antibody

Catalog Number:

11291

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q13480
NCBI Protein: NP_002030.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 627 (V-E-Y(p)-L-D) derived from Human Gab1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Gab1 only when phosphorylated at tyrosine 627.

Applications:

WB IF

Background:

The protein encoded by Gab1 is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.

Anders Kallin, et al. (2004) J. Biol. Chem ; 279: 17897 - 17904.
Hideto Kameda, et al. (2001) Cell Growth Differ ; 12: 307.
Masaki Osawa, et al. (2002) J. Cell Biol ; 158: 773.

References:

appl_detail:

Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Datasheet PDF