FAK(Phospho-Tyr397) Antibody

Catalog Number:

11215

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q05397
NCBI Protein: NP_001186578.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 397(D-D-Y(p)-A-E)derived from Human FAK.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of FAK only when phosphorylated at Tyrosine 397.

Applications:

WB

Background:

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Matsuya M.et.al. (1998)J. Biol. Chem. 273:1003-1014
Fujita H.et.al. (1998)J. Biol. Chem. 273:26516-26521
Le Romancer M.et.al. (2008)Mol. Cell 31:212-221

References:

appl_detail:

Predicted MW: 125kd
Western blotting: 1:500~1:1000

Datasheet PDF