Product Datasheet  
FAK(Phospho-Tyr397) Antibody  
Catalog Number: 11215  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q05397
    NCBI Protein: NP_001186578.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 397(D-D-Y(p)-A-E)derived from Human FAK.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 125kd
    Western blotting: 1:500~1:1000
  • other_names：  
  • FAK; FAK1; PTK2
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of FAK only when phosphorylated at Tyrosine 397.
  • Applications：  
  • WB
  • Background：  
  • Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

    Matsuya M.et.al. (1998)J. Biol. Chem. 273:1003-1014
    Fujita H.et.al. (1998)J. Biol. Chem. 273:26516-26521
    Le Romancer M.et.al. (2008)Mol. Cell 31:212-221



 
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