IRS-1(Phospho-Ser312) Antibody

Catalog Number:

11143

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P35568
NCBI Protein: NP_005535.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 312 (A-T-S(p)-P-A) derived from Human IRS-1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 312.

Applications:

WB

Background:

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit

Tzatsos A, et al. (2006) Mol Cell Biol; 26(1): 63-76
Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645
Szanto I, et al. (2000) Proc Natl Acad Sci U S A; 97(5): 2355-2360
Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645

References:

appl_detail:

Predicted MW: 180kd
Western blotting: 1:500~1:1000

Datasheet PDF