Product Datasheet  
IRS-1(Phospho-Ser312) Antibody  
Catalog Number: 11143  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P35568
    NCBI Protein: NP_005535.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine 312 (A-T-S(p)-P-A) derived from Human IRS-1.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 180kd
    Western blotting: 1:500~1:1000
  • other_names：  
  • HIRS-1; IRS1; Insulin receptor substrate 1
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 312.
  • Applications：  
  • WB
  • Background：  
  • May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit

    Tzatsos A, et al. (2006) Mol Cell Biol; 26(1): 63-76
    Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645
    Szanto I, et al. (2000) Proc Natl Acad Sci U S A; 97(5): 2355-2360
    Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645



 
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