HNF4a(Phospho-Ser304) Antibody

Catalog Number:

11043

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P41235
NCBI Protein: NP_000448.3

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 304 (L-R-S(p)-Q-V)derived from Human HNF4a.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of HNF4a only when phosphorylated at serine 304.

Applications:

WB

Background:

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes,including hepatocyte nuclear factor 1 a, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

Kritis A.A,et al.Gene 173:275-280(1996)
Drewes T,et al.Mol. Cell. Biol. 16:925-931(1996)
Yamagata K,et al.Nature 384:458-460(1996)
Tanaka T,et al.Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases

References:

appl_detail:

Predicted MW: 52kd
Western blotting: 1:1000

Datasheet PDF