Smad3 (Phospho-Ser423/425) Antibody

Catalog Number:

13335

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P84022

Form of Antibody:

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage/Stability:

Immunogen:

peptide

Purification:

Immunogen affinity purified

Specificity/Sensitivity:

Applications:

WB, IP, IF, IHC

Background:

Smad proteins, the mammalian homologs of the Drosophila mothers against dpp (Mad), mediate intracellular signaling of the TGFβ superfamily. TGFβ type I receptor mediates phosphorylation of Smad2 (also designated Madr2 or JV18-1) at the C-terminal Ser 465 and Ser 467 residues, and phosphorylation of Smad3 at the C-terminal Ser 423 and Ser 425 residues in an obligate order. Upon phosphorylation and activation by the active TGFβ receptor complex,Smad2 and Smad3 homo-oligomerize or hetero-oligomerize with Smad4,and translocate into the nucleus. There they interact with various cellular partners, bind DNA, regulate transcription and cross-talk with other signaling pathways. Activin A also induces these cellular responses by phosphorylating Smad2, but it is a less potent activator than TGFβ. TGFβ-induced phosphorylation of Smad3 promotes its interaction with coactivators CBP and p300 and is essential for the TGFβ-mediated inhibition of cellular proliferation.

References:

appl_detail:

WB: 1:1,000
IHC: 1:50-500

IP: 1-2 μg per 100-500 μg of total protein

Datasheet PDF