Product Datasheet  
Smad3 (Phospho-Ser423/425) Antibody  
Catalog Number: 13335  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Goat
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#:P84022
  • Form of Antibody：  
  • 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • peptide
  • reactivity：  
  • Hu, Ms, Rt
  • appl_detail：  
  • WB: 1:1,000
    IHC: 1:50-500

    IP: 1-2 μg per 100-500 μg of total protein
  • other_names：  
  • hMAD-3 antibody
    hSMAD3 antibody
    HSPC193 antibody
    HsT17436 antibody
    JV15-2 antibody
    LDS1C antibody
    MAD homolog 3 antibody
    Mad homolog JV15 2 antibody
    MAD mothers against decapentaplegic homolog 3 antibody
    Mad pr
  • Purification：  
  • Immunogen affinity purified
  • Specificity：  
  • Applications：  
  • WB, IP, IF, IHC
  • Background：  
  • Smad proteins, the mammalian homologs of the Drosophila mothers against dpp (Mad), mediate intracellular signaling of the TGFβ superfamily. TGFβ type I receptor mediates phosphorylation of Smad2 (also designated Madr2 or JV18-1) at the C-terminal Ser 465 and Ser 467 residues, and phosphorylation of Smad3 at the C-terminal Ser 423 and Ser 425 residues in an obligate order. Upon phosphorylation and activation by the active TGFβ receptor complex,Smad2 and Smad3 homo-oligomerize or hetero-oligomerize with Smad4,and translocate into the nucleus. There they interact with various cellular partners, bind DNA, regulate transcription and cross-talk with other signaling pathways. Activin A also induces these cellular responses by phosphorylating Smad2, but it is a less potent activator than TGFβ. TGFβ-induced phosphorylation of Smad3 promotes its interaction with coactivators CBP and p300 and is essential for the TGFβ-mediated inhibition of cellular proliferation.



 
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