C/EBP-ε (Phospho-Thr74) Antibody

Catalog Number:

11686

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q15744;
NCBI Gene#: 1053;
NCBI Protein#: NP_001796.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 174 (L-A-T(p)-A-A) derived from Human C/EBP-ε.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of C/EBP-ε only when phosphorylated at threonine 74

Applications:

WB

Background:

C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

Arati Khanna-Gupta. PNAS, Jul 2001; 98: 8000.
Dazhong Zhuang. J. Biol. Chem., Apr 2006; 281: 10745 - 10751.
Julie A. Lekstrom-Himes. Stem Cells, Mar 2001; 19: 125 - 133.
Ryuya Yamanaka. PNAS, Nov 1997; 94: 13187.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF