Product Datasheet  
C/EBP-ε (Phospho-Thr74) Antibody  
Catalog Number: 11686  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: Q15744;
    NCBI Gene#: 1053;
    NCBI Protein#: NP_001796.2.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 174 (L-A-T(p)-A-A) derived from Human C/EBP-ε.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:500~1:1000

  • other_names：  
  • CEBPE; C/EBP epsilon; CCAAT/enhancer binding protein epsilon;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of C/EBP-ε only when phosphorylated at threonine 74
  • Applications：  
  • WB
  • Background：  

  • C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

    Arati Khanna-Gupta. PNAS, Jul 2001; 98: 8000.
    Dazhong Zhuang. J. Biol. Chem., Apr 2006; 281: 10745 - 10751.
    Julie A. Lekstrom-Himes. Stem Cells, Mar 2001; 19: 125 - 133.
    Ryuya Yamanaka. PNAS, Nov 1997; 94: 13187.




 
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