Estrogen Receptor-α (Phospho-Ser102) Antibody

Catalog Number:

11801

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P03372;
NCBI Gene#: 2099;
NCBI Protein#: NP_000116.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine102(L-N-S(p)-V-S) derived from Human Estrogen Receptor-α.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Estrogen Receptor-α only when phosphorylated at serine 102.

Applications:

WB IHC

Background:

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

Greene G.L., Science 231:1150-1154(1986).
Green S., Nature 320:134-139(1986).
Pink J.J., Nucleic Acids Res. 24:962-969(1996).

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF