Ras-GRF1 (Phospho-Ser916) Antibody

Catalog Number:

11678

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q13972;
NCBI Gene#: 5923;
NCBI Protein#: NP_002882.3.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 916(R-M-S(p)-L-A) derived from Mouse Ras-GRF1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at serine 916.

Applications:

IHC

Background:

Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.

Huibin Yang, J. Biol. Chem., Apr 2003; 278: 13278 - 13285.
Huibin Yang, Mol. Biol. Cell, May 2006; 17: 2177 - 2189.
Raymond R. Mattingly, J. Biol. Chem., Dec 1999; 274: 37379.
Tie-Nian Zhu, J. Biol. Chem., May 2007; 282: 14816 - 14826.

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

Datasheet PDF