Product Datasheet  
Ras-GRF1 (Phospho-Ser916) Antibody  
Catalog Number: 11678  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: Q13972;
    NCBI Gene#: 5923;
    NCBI Protein#: NP_002882.3.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Serine 916(R-M-S(p)-L-A) derived from Mouse Ras-GRF1.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  

  • Immunohistochemistry: 1:50~1:100

  • other_names：  
  • CDC25; GNRP; GRF1; RASGRF1;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at serine 916.
  • Applications：  
  • IHC
  • Background：  

  • Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.

    Huibin Yang, J. Biol. Chem., Apr 2003; 278: 13278 - 13285.
    Huibin Yang, Mol. Biol. Cell, May 2006; 17: 2177 - 2189.
    Raymond R. Mattingly, J. Biol. Chem., Dec 1999; 274: 37379.
    Tie-Nian Zhu, J. Biol. Chem., May 2007; 282: 14816 - 14826.




 
© Signalway Biotechnology All Rights Reserved.