Smad2(Phospho-Thr220) Antibody

Catalog Number:

11323

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q15796
NCBI Protein: NP _001003652.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 220 (P-E-T(p)-P-P) derived from Human Smad2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Smad2 only when phosphorylated at threonine 220.

Applications:

WB

Background:

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15(2): 420

References:

appl_detail:

Predicted MW: 60kd
Western blotting: 1:500~1:1000

Datasheet PDF