Product Datasheet  
Smad2(Phospho-Thr220) Antibody  
Catalog Number: 11323  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q15796
    NCBI Protein: NP _001003652.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 220 (P-E-T(p)-P-P) derived from Human Smad2.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 60kd
    Western blotting: 1:500~1:1000
  • other_names：  
  • JV18-1; MADH2; MADR2; Mad-related protein 2; Mothers against DPP homolog 2
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of Smad2 only when phosphorylated at threonine 220.
  • Applications：  
  • WB
  • Background：  
  • Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

    Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15(2): 420



 
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