Smad1(Phospho-Ser465) Antibody

Catalog Number:

11321

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q15797
NCBI Protein: NP_001003688.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 465 (I-S-S-V-S(p)) derived from Human Smad1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Smad1 only when phosphorylated at serine 465.

Applications:

WB

Background:

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).

Hiroaki Seto, et al. (2004) J Clin Invest. March 1; 113(5): 718

References:

appl_detail:

Predicted MW: 55kd
Western blotting: 1:500~1:1000

Datasheet PDF