SHP-2(Phospho-Tyr580) Antibody

Catalog Number:

11320

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q06124
NCBI Protein: NP_002825.3

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 580 (R-V-Y(p)-E-N) derived from Human SHP-2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of SHP-2 only when phosphorylated at tyrosine 580.

Applications:

WB IHC

Background:

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.

Ferjoux G, et al. (2003) Mol Biol Cell. 2003 ; 14(9): 3911-3928.
Shi ZQ, et al. (2000) Mol Cell Biol ; 20(5): 1526-1536.
Li C, Friedman JM. (1999) Proc Natl Acad Sci U S A ; 96(17): 9677-9682
Manes S, et al. (1999) Mol Cell Biol ; 19(4): 3125-3135.
Oh ES, et al. (1999) Mol Cell Biol; 19(4): 3205-3215.
 

References:

appl_detail:

Predicted MW: 72kd
Immunohistochemistry: 1:50~1:100
 

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