Anti-Taq DNA Polymerase Antibody

Catalog Number:

48369

Amount:

100μg

Price:

$529

Swiss-Prot No:

Form of Antibody:

Storage/Stability:

Immunogen:

Taq DNA Polymerase

Purification:

ProG affinity purified

Specificity/Sensitivity:

Applications:

Antibody-mediated hot start PCR

Background:

Polymerase Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who was awarded the 1993 Nobel Prize for chemistry for PCR, is a technique to exponentially amplify in vitro a small quantity of a specific nucleotide sequence using a thermostable (Taq) DNA polymerase. Anti-Taq Antibody is an ideal tool for hot-start PCR with Taq DNA polymerase. The Anti-Taq Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing non-specific and primer dimer amplification resulted from non-specific priming at ambient temperature for the duration of time prior to PCR thermal cycling. During the initial denaturing step in PCR thermal cycling, the Anti-Taq Antibody is denatured and the Taq DNA polymerase is then released, thus regaining its full DNA polymerase activity. The result indicates that anti-Taq DNA Polymerase antibody increases the specificity and sensitivity of the PCR.

References:

appl_detail:

Datasheet PDF