Product Datasheet  
Anti-Taq DNA Polymerase Antibody  
Catalog Number: 48369  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Mouse
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Form of Antibody：  
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Taq DNA Polymerase
  • reactivity：  
  • appl_detail：  



  •  

  • other_names：  
  • Purification：  
  • ProG affinity purified
  • Specificity：  
  • Applications：  
  • Antibody-mediated hot start PCR
  • Background：  

  • Polymerase Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who was awarded the 1993 Nobel Prize for chemistry for PCR, is a technique to exponentially amplify in vitro a small quantity of a specific nucleotide sequence using a thermostable (Taq) DNA polymerase. Anti-Taq Antibody is an ideal tool for hot-start PCR with Taq DNA polymerase. The Anti-Taq Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing non-specific and primer dimer amplification resulted from non-specific priming at ambient temperature for the duration of time prior to PCR thermal cycling. During the initial denaturing step in PCR thermal cycling, the Anti-Taq Antibody is denatured and the Taq DNA polymerase is then released, thus regaining its full DNA polymerase activity. The result indicates that anti-Taq DNA Polymerase antibody increases the specificity and sensitivity of the PCR.




 
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