ALAS2 Antibody

Catalog Number:

48271

Amount:

100μg

Price:

$529

Swiss-Prot No:

Swiss-Prot#:P22557

Form of Antibody:

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage/Stability:

Immunogen:

peptide

Purification:

ProA affinity purified

Specificity/Sensitivity:

Applications:

WB, IP, IF, IHC(P)

Background:

5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia.

References:

appl_detail:

WB: 1:100-1:1,000
IHC: 1:50-500

IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)

Datasheet PDF