Flag-Tag Mouse Monoclonal Antibody

Catalog Number:

Amount:

100μg

Price:

$529

Swiss-Prot No:

Form of Antibody:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Purification:

Affinity purification using immunogen.

Specificity/Sensitivity:

The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion?proteins.

Applications:

WB,IF IP

Background:

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

References:

appl_detail:

Western blotting: 1:5000~1:10000
Immunofluorescence: 1:2000
Immunoprecipitation: 1:5000

Datasheet PDF