Product Datasheet  
Flag-Tag Mouse Monoclonal Antibody  
Catalog Number:  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Mouse
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Form of Antibody：  
  • Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  

  • Western blotting: 1:5000~1:10000
    Immunofluorescence: 1:2000
    Immunoprecipitation: 1:5000

  • other_names：  
  • Purification：  
  • Affinity purification using immunogen.
  • Specificity：  
  • The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion?proteins.
  • Applications：  
  • WB,IF IP
  • Background：  

  • The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.




 
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