PARP1 antibody

Catalog Number:

38592

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P09874
NCBI Gene ID: 142

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Recombinant protein of human PARP1.

Purification:

Antibodies were purified by affinity purification using immunogen.

Specificity/Sensitivity:

The antibody detects endogenous level of total PARP1 antibody.

Applications:

WB IHC IF

Background:

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

References:

appl_detail:

Western blotting: 1:500 - 1:1000
Immunohistochemistry: 1:50 - 1:100
Immunofluorescence: 1:50 - 1:100

Datasheet PDF