Product Datasheet  
PARP1 antibody  
Catalog Number: 38592  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P09874
    NCBI Gene ID: 142
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Recombinant protein of human PARP1.
  • reactivity：  
  • Hu Ms
  • appl_detail：  
  • Western blotting: 1:500 - 1:1000
    Immunohistochemistry: 1:50 - 1:100
    Immunofluorescence: 1:50 - 1:100
  • other_names：  
  • ADPRT; ADPRT1; PARP; PARP-1; PPOL; pADPRT-1;
  • Purification：  
  • Antibodies were purified by affinity purification using immunogen.
  • Specificity：  
  • The antibody detects endogenous level of total PARP1 antibody.
  • Applications：  
  • WB IHC IF
  • Background：  
  • PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).



 
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