MARCKS(Phospho-Ser162) Antibody

Catalog Number:

11265

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P29966
NCBI Protein: NP_002347.5

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 162 (K-K-S(p)-F-K) derived from Human MARCKS.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of MARCKS only when phosphorylated at serine 162.

Applications:

WB IF

Background:

MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102(35): 12407-12412
Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280(3): 605-609
Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359(2): 151-159

References:

appl_detail:

Predicted MW: 80kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Datasheet PDF