Product Datasheet  
MARCKS(Phospho-Ser162) Antibody  
Catalog Number: 11265  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P29966
    NCBI Protein: NP_002347.5
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine 162 (K-K-S(p)-F-K) derived from Human MARCKS.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 80kd
    Western blotting: 1:500~1:1000
    Immunofluorescence: 1:100~1:200
  • other_names：  
  • MACS; MARCS; PKCSL; PRKCSL; Protein kinase C substrate
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of MARCKS only when phosphorylated at serine 162.
  • Applications：  
  • WB IF
  • Background：  
  • MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

    Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102(35): 12407-12412
    Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280(3): 605-609
    Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359(2): 151-159



 
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