p27Kip1(Phospho-Thr187) Antibody

Catalog Number:

11208

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P46527
NCBI Protein: NP_004055.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 187 (E-Q-T(p)-P-K) derived from Human p27Kip1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of p27Kip1 only when phosphorylated at threonine 187.

Applications:

WB IHC

Background:

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.

Eguchi H, et al. (2003) Cancer Res; 63(15): 4739-46
Le XF, et al. (2003) J Biol Chem;
Connor MK, et al. (2003)

References:

appl_detail:

Predicted MW: 27kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF