Product Datasheet  
p27Kip1(Phospho-Thr187) Antibody  
Catalog Number: 11208  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P46527
    NCBI Protein: NP_004055.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 187 (E-Q-T(p)-P-K) derived from Human p27Kip1.
  • reactivity：  
  • Hu
  • appl_detail：  
  • Predicted MW: 27kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
  • other_names：  
  • CDKN1B; CDN1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; KIP1
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of p27Kip1 only when phosphorylated at threonine 187.
  • Applications：  
  • WB IHC
  • Background：  
  • Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.

    Eguchi H, et al. (2003) Cancer Res; 63(15): 4739-46
    Le XF, et al. (2003) J Biol Chem;
    Connor MK, et al. (2003)



 
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