p21Cip1(Phospho-Thr145) Antibody

Catalog Number:

11206

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P38936
NCBI Protein: NP _000380.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 145 (R-Q-T(p)-S-M) derived from Human p21Cip1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of p21Cip1 only when phosphorylated at threonine 145.

Applications:

IHC

Background:

May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.

Ocker M Int,et al. (2007)J Biochem Cell Biol. 39(7-8):1367-74.
Dangi S, et al.(2006)Cell Prolif. 39(4):261-79.
Chen J,et al.(2006)Am J Physiol Heart Circ Physiol. 290(4):H1575-86

References:

appl_detail:

Predicted MW: 21kd
Immunohistochemistry: 1:50~1:100

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