Product Datasheet  
p21Cip1(Phospho-Thr145) Antibody  
Catalog Number: 11206  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P38936
    NCBI Protein: NP _000380.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 145 (R-Q-T(p)-S-M) derived from Human p21Cip1.
  • reactivity：  
  • Hu
  • appl_detail：  
  • Predicted MW: 21kd
    Immunohistochemistry: 1:50~1:100
  • other_names：  
  • CAP20; CDK-interacting protein 1; CDKN1; CDKN1A; CDN1A
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of p21Cip1 only when phosphorylated at threonine 145.
  • Applications：  
  • IHC
  • Background：  
  • May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.

    Ocker M Int,et al. (2007)J Biochem Cell Biol. 39(7-8):1367-74.
    Dangi S, et al.(2006)Cell Prolif. 39(4):261-79.
    Chen J,et al.(2006)Am J Physiol Heart Circ Physiol. 290(4):H1575-86



 
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