14-3-3z(Phospho-Ser58) Antibody

Catalog Number:

11181

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P63104
NCBI Protein: NP_001129171.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 58 (R-S-S(p)-W-R) derived from Human 14-3-3 zeta.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of 14-3-3 z only when phosphorylated at serine 58.

Applications:

WB IHC IF

Background:

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

Gu YM, et al. (2006) FEBS Lett ; 580(1): 305-310
Powell DW, et al. (2003) Mol Cell Biol; 23(15): 5376-5387
Mackintosh C. (2004) Biochem J; 381(Pt 2): 329-342.

References:

appl_detail:

Predicted MW: 28kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Datasheet PDF