Product Datasheet  
14-3-3z(Phospho-Ser58) Antibody  
Catalog Number: 11181  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P63104
    NCBI Protein: NP_001129171.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine 58 (R-S-S(p)-W-R) derived from Human 14-3-3 zeta.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 28kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200
  • other_names：  
  • 1433Z; 143Z; FAS; Factor activating exoenzyme S; KCIP-1
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of 14-3-3 z only when phosphorylated at serine 58.
  • Applications：  
  • WB IHC IF
  • Background：  
  • This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

    Gu YM, et al. (2006) FEBS Lett ; 580(1): 305-310
    Powell DW, et al. (2003) Mol Cell Biol; 23(15): 5376-5387
    Mackintosh C. (2004) Biochem J; 381(Pt 2): 329-342.



 
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