eNOS(Phospho-Ser1177) Antibody

Catalog Number:

11156

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P29474
NCBI Protein: NP_000594.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 1177 (T-Q-S(p)-F-S) derived from Human eNOS.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of eNOS only when phosphorylated at serine 1177.

Applications:

WB

Background:

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Fulton, D. et al. (1999) Nature 399, 597-601.
Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.

References:

appl_detail:

Predicted MW: 133kd

Western blotting: 1:500~1:1000

Datasheet PDF