Product Datasheet  
eNOS(Phospho-Ser1177) Antibody  
Catalog Number: 11156  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P29474
    NCBI Protein: NP_000594.2
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine 1177 (T-Q-S(p)-F-S) derived from Human eNOS.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  

  • Predicted MW: 133kd

    Western blotting: 1:500~1:1000

  • other_names：  
  • Constitutive NOS; EC-NOS; ECNOS; NOS3; NOSIII
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of eNOS only when phosphorylated at serine 1177.
  • Applications：  
  • WB
  • Background：  

  • Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

    Fulton, D. et al. (1999) Nature 399, 597-601.
    Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
    Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.




 
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