cofilin(Phospho-Ser3) Antibody

Catalog Number:

11139

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P23528
NCBI Protein: NP_005498.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from Human cofilin.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of cofilin only when phosphorylated at serine 3.

Applications:

WB IHC IF

Background:

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Kobayashi M, et al. (2006) EMBO J 25(4): 713-26.
Wang Y, et al. (2005) Biol Chem 280(13): 12683-9.
Smith-Beckerman DM, et al. (2005) Mol Cell Proteomics: 156-68.

References:

appl_detail:

Predicted MW: 19kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Datasheet PDF