Product Datasheet  
cofilin(Phospho-Ser3) Antibody  
Catalog Number: 11139  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P23528
    NCBI Protein: NP_005498.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from Human cofilin.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  

  • Predicted MW: 19kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200

  • other_names：  
  • CFL; CFL1;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of cofilin only when phosphorylated at serine 3.
  • Applications：  
  • WB IHC IF
  • Background：  

  • Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

    Kobayashi M, et al. (2006) EMBO J 25(4): 713-26.
    Wang Y, et al. (2005) Biol Chem 280(13): 12683-9.
    Smith-Beckerman DM, et al. (2005) Mol Cell Proteomics: 156-68.




 
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