LIMK2(Phospho-Thr505) Antibody

Catalog Number:

11127

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P53671
NCBI Protein: NP_001026971.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 505 (R-Y-T(p)-V-V) derived from Human LIMK2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of LIMK2 only when phosphorylated at threonine 505.

Applications:

WB IHC IF

Background:

Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.

Soosairajah J, et al. (2005) EMBO J.
Ohashi K, et al. (2000) J Biol Chem; 275(5): 3577-82.
Edwards DC, et al. (1999) Nat Cell Biol; 1(5): 253-9.

References:

appl_detail:

Predicted MW: 70kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Datasheet PDF