Product Datasheet  
LIMK2(Phospho-Thr505) Antibody  
Catalog Number: 11127  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P53671
    NCBI Protein: NP_001026971.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 505 (R-Y-T(p)-V-V) derived from Human LIMK2.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 70kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200
  • other_names：  
  • LIMK-2; kinase LIMK2;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of LIMK2 only when phosphorylated at threonine 505.
  • Applications：  
  • WB IHC IF
  • Background：  
  • Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.

    Soosairajah J, et al. (2005) EMBO J.
    Ohashi K, et al. (2000) J Biol Chem; 275(5): 3577-82.
    Edwards DC, et al. (1999) Nat Cell Biol; 1(5): 253-9.



 
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